| 1. | Differentiation of chicken primordial germ cells in vitro
鸡胚原始生殖细胞体外的分化 |
| 2. | Isolation and long term culture of human primordial germ cells
人原始生殖细胞的分离和体外培养 |
| 3. | Studies on isolation , culture and biological charactristics of buffalo primordial germ cells
的分离培养及生物学特性的研究 |
| 4. | The alkaline phosphatase activity test showed these cells maintained the character of primordial germ cells
碱性磷酸酶( akp )活性检测结果证实了这些细胞集落确实具有原始生殖嵴干细胞的特性。 |
| 5. | Recently , primordial germ cells ( pgcs ) , which are the progenitors of the sperm or egg cells , have provided an alternative source of pluripotent stem cells
近年来，作为精子和卵子的祖细胞的原始生殖细胞( pgcs )已成为另一种可选择的多能性干细胞。 |
| 6. | Inner cell mass ( icm ) of the blastocyst stage embryo and primordial germ cells ( pgcs ) of fetus are the mostly used materials for derivation of pluripotent stem cells
早期囊胚的内细胞团( innercellmass ， icm )和原始生殖细胞( primordialgermcells ， pgcs )是获得多能干细胞( pluripotentstemcell )的两种主要的材料来源。 |
| 7. | 2 . isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8 . 5 - 15 . 5 dpc ( days post coitum ) embryos . eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage
Icr小鼠eg细胞的分离克隆研究本试验以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc为材料，经传代培养，获得能连续而稳定传至6代的，具有胚胎干细胞诸多特性的eg细胞系。 |
| 8. | ( 3 ) isolation and culture of human primordial germ cells ( pgcs ) . human pgcs collected from gonadal ridges and mesenteries were grown on mouse feeder layers in the presence of human recombinant leukemia inhibitory factor ( lif ) , human recombinant basic fibroblast growth factor , and forskolin as described previously . initially , pgcs were visualized by alkaline phosphatase activity staining
( 3 )人类pgcs的分离和培养从4 10周龄药物流产胚胎的生殖嵴和肠系膜组织中分离原始生殖细胞( primordialgermcells ， pgcs ) ，培养在添加人重组白血病抑制因子( lif ) 、人重组碱性成纤维细胞生长因子( bfgf )和forskolin的小鼠饲养层细胞上。 |
| 9. | Embryonic stem ( es ) cells were derived from the inner cell mass ( icm ) of preimplantation embryos , and embryonic germ ( eg ) cells from primordial germ cells ( pgc ) . both es and eg cells were cultured to determine the factors affecting on isolation , cloning and passage of pluripotent stem cells from icr mouse embryos
试验以icr小鼠胚胎内细胞团( icm )和原始生殖细胞( pgc )为材料，采用不同培养体系分离克隆胚胎多能干细胞，探讨icr品系小鼠胚胎多能干细胞分离克隆的基本方法，以及一些因素对其分离、培养、克隆、传代等效果的影响。 |
| 10. | The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy . after mechanical desection and enzymic digestion , the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin . the medium contains several cytokines : lif ( leukemia inhibitory factor ) , bfgf and foskolin
从4 ? 8周的流产胎儿的原始生殖嵴部位分离到原始生殖细胞，经过机械和化学方法的解离后培养于事先用丝裂霉素处理过的小鼠胚胎成纤维细胞或人胚胎成纤维细胞饲养层上，培养基中添加了lif ， bfgef ， foskolin等细胞因子，此后大约每7天左右传代一次。 |
